BMG LABTECH experts Ann-Cathrin Volz and Martin Mangold discuss microplate readers and their usage recommendations. BMG LABTECH specializes in microplate readers with various models to meet researchers’ needs. They offer single-mode to multi-mode instruments, including the CLARIOstar® Plus and SPECTROstar® Nano, each designed for specific research requirements. The choice of microplate color impacts measurements in absorbance, fluorescence, and luminescence assays. The optimal excitation and emission wavelengths for a fluorophore can be determined by examining their spectra. Adjustments in gain setting, integration time, and scan options play a crucial role in microplate measurements. The experts also address autofluorescence and the benefits of bottom optics in reducing its negative effects.
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