Mitochondrial calcium signaling plays a critical role in energy metabolism and calcium balance, but accurate monitoring is difficult due to pH fluctuations. Existing fluorescent calcium indicators are sensitive to pH changes, leading to inaccuracies in measurements. TurCaMP, a new GECI developed from mTurquoise2, offers an inverse response to calcium transients and is pH insensitive within the physiological range. This allows for high-quality imaging of mitochondrial calcium signaling with minimal background noise. TurCaMP’s cyan fluorescence also enables multiplexed imaging of calcium signals in different cellular compartments, providing new insights into complex physiological processes. This advancement showcases the potential of computational modeling and experimental validation in developing innovative calcium imaging tools.
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Novel tool holds great promise for studying mitochondrial calcium signaling
