Boosting protein stability and resilience with interlocking rings

Prof. Wen-Bin Zhang and Dr. Jing Fang led a study on a single-domain protein catenane, which is a rare structure in nature. They achieved this design by introducing artificial entanglement in the structure and synthesizing it through post-translational processing events in cells. The cat-DHFR, a single-domain catenane, was thoroughly characterized and found to have enhanced anti-aggregation properties and better thermal resilience compared to the linear control. The research team believes that this method could be applicable to other single-domain proteins, opening up new possibilities for protein molecules with functional benefits of topology. This new class of protein molecules has great potential for a broad range of applications.

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