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Functional Applications of Stable Tau Oligomers in Cell Biology and Electrophysiology Studies



Hill, Emily;

Moffat, Kevin G;

Wall, Mark J;

Zetterberg, Henrik;

Blennow, Kaj;

Karikari, Thomas K;

(2022)

Functional Applications of Stable Tau Oligomers in Cell Biology and Electrophysiology Studies.

Protein Aggregation : Methods in Molecular Biology
, 2551

pp. 147-161.

10.1007/978-1-0716-2597-2_11.


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Abstract

Aggregated tau protein plays a key role in the pathogenesis of neurodegenerative tauopathies including Alzheimer’s disease. Soluble, low-molecular-weight tau oligomers are formed early in disease processes and are thought to have toxic functions that disrupt neuronal function. The dynamic and transient nature of tau oligomers complicates in vitro functional studies to explore the mechanistic links between oligomer formation and neurodegeneration. We have previously described a method of producing stable and structurally characterized oligomers that maintain their oligomeric conformation and prevent further aggregation. This method allows for the flexibility of stabilizing tau oligomers by specifically labelling cysteine residues with fluorescent or colorless maleimide conjugates. Here, we describe the functional applications of these preformed stable tau oligomers in cell biology and electrophysiological studies. These investigations allow real-time insights into the cellular uptake of exogenous tau oligomers and their functional effects in the recipient cells.

Type: Article

Title: Functional Applications of Stable Tau Oligomers in Cell Biology and Electrophysiology Studies
Location: United States
DOI: 10.1007/978-1-0716-2597-2_11
Publisher version: https://doi.org/10.1007/978-1-0716-2597-2_11
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Alzheimer Disease, Electrophysiology, Humans, Neurons, tau Proteins, Tauopathies, Alzheimer’s disease, Electrophysiology, Oligomer, Tau, Tauopathies
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Neurodegenerative Diseases
URI: https://discovery.ucl.ac.uk/id/eprint/10161427
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