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Amelanotic B16-F10 melanoma compatible with advanced 3-D imaging modalities



Advanced 3D-imaging of the immune system within the melanoma tumour microenvironment holds tremendous implications. The most commonly used syngeneic model for understanding melanoma pathophysiology employs the transplantation of the B16-F10 melanoma cell line into mouse skin/hypodermis (Guo et al., 2018, Overwijk and Restifo, 2001, Potez et al., 2018). However, B16-F10 cells are heavily pigmented, which impedes the penetration of light into solid tumours. Additionally, the presence of melanin may induce “speckling” when 2-photon laser light hits melanoma cells, resulting in compromised image quality (Roediger et al., 2008).

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